Insensitivity of bacteria to proposed antimetabolites of D-alanyl-D-alanine.
نویسندگان
چکیده
alpha-Aminoisobutyryl-d-alanine, d-alanyl-alpha-aminoisobutyric acid, and alpha-amino-isobutyryl-alpha-aminoisobutyric acid, proposed as antimetabolites of d-alanyl-d-alanine in bacterial cell wall synthesis, failed to inhibit bacterial growth.
منابع مشابه
Docking studies in target proteins involved in antibacterial action mechanisms: extending the knowledge on standard antibiotics to antimicrobial mushroom compounds.
In the present work, the knowledge on target proteins of standard antibiotics was extended to antimicrobial mushroom compounds. Docking studies were performed for 34 compounds in order to evaluate their affinity to bacterial proteins that are known targets for some antibiotics with different mechanism of action: inhibitors of cell wall synthesis, inhibitors of protein synthesis, inhibitors of n...
متن کاملDdlN from vancomycin-producing Amycolatopsis orientalis C329.2 is a VanA homologue with D-alanyl-D-lactate ligase activity.
Vancomycin-resistant enterococci acquire high-level resistance to glycopeptide antibiotics through the synthesis of peptidoglycan terminating in D-alanyl-D-lactate. A key enzyme in this process is a D-alanyl-D-alanine ligase homologue, VanA or VanB, which preferentially catalyzes the synthesis of the depsipeptide D-alanyl-D-lactate. We report the overexpression, purification, and enzymatic char...
متن کاملModifications of the acyl-D-alanyl-D-alanine terminus affecting complex-formation with vancomycin.
Vancomycin forms complexes with peptides terminating in d-alanyl-d-alanine that are analogous to the biosynthetic precursors of bacterial mucopeptides. The specificity of complex-formation has been studied by means of many synthetic peptides, prepared by both solid-phase and conventional methods. The following conclusions can be drawn: (a) three amide linkages are required to form a stable comp...
متن کاملAntagonisms in the utilization of d-amino acids by lactic acid bacteria. IV. d-Aspartic acid.
Investigations with glutamic acid, methionine, and alanine have shown that n-amino acid utilization by lactic acid bacteria may be inhibited by specific antimetabolites (l-4). This has been taken to indicate that a conversion of o-amino acid substrate to the L form precedes its utilization, and that this process may be blocked by an appropriate inhibitor. Both Land n-alanines have been shown to...
متن کاملRole of the D-alanyl carrier protein in the biosynthesis of D-alanyl-lipoteichoic acid.
D-Alanyl-lipoteichoic acid (D-alanyl-LTA) is a widespread macroamphiphile which plays a vital role in the growth and development of gram-positive organisms. The biosynthesis of this polymer requires the enzymic activation of D-alanine for its transfer to the membrane-associated LTA (mLTA). A small, heat-stable, and acidic protein that is required for this transfer was purified to greater than 9...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 114 1 شماره
صفحات -
تاریخ انتشار 1973